Glucose transport engineering allows mimicking fed-batch performance in batch mode and selection of superior producer strains.

ABSTRACT:

Background

Fed-batch mode is the standard culture technology for industrial bioprocesses. Nevertheless, most of the early-stage cell and process development is carried out in batch cultures, which can bias the initial selection of expression systems. Cell engineering can provide an alternative to fed-batch cultures for high-throughput screening and host selection. We have previously reported a library of Escherichia coli strains with single and multiple deletions of genes involved in glucose transport. Compared to their wild type (W3110), the mutant strains displayed lower glucose uptake, growth and aerobic acetate production rates. Therefore, when cultured in batch mode, such mutants may perform similar to W3110 cultured in fed-batch mode. To test that hypothesis, we evaluated the

SUBMITTER: Velazquez D 

PROVIDER: S-EPMC9450411 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature