Neoantigen-based cancer vaccination using chimeric RNA-loaded dendritic cell-derived extracellular vesicles.

ABSTRACT: Cancer vaccines critically rely on the availability of targetable immunogenic cancer-specific neoepitopes. However, mutation-based immunogenic neoantigens are rare or even non-existent in subgroups of cancer types. To address this issue, we exploited a cancer-specific aberrant transcription-induced chimeric RNA, designated A-P_as chiRNA, as a possible source of clinically relevant and targetable neoantigens. A-P_as chiRNA encodes a recently discovered cancer-specific chimeric protein that comprises full-length astrotactin-2 (ASTN2) C-terminally fused in-frame to the antisense sequence of the 18^th intron of pregnancy-associated plasma protein-A (PAPPA). We used extracellular vesicles (EVs) from A-P_as chiRNA-transfected dendritic cells (DCs) to produ

SUBMITTER: Xiong X 

PROVIDER: S-EPMC9451527 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature