In vivo single-molecule analysis reveals COOLAIR RNA structural diversity.

ABSTRACT: Cellular RNAs are heterogeneous with respect to their alternative processing and secondary structures, but the functional importance of this complexity is still poorly understood. A set of alternatively processed antisense non-coding transcripts, which are collectively called COOLAIR, are generated at the Arabidopsis floral-repressor locus FLOWERING LOCUS C (FLC)¹. Different isoforms of COOLAIR influence FLC transcriptional output in warm and cold conditions^2-7. Here, to further investigate the function of COOLAIR, we developed an RNA structure-profiling method to determine the in vivo structure of single RNA molecules rather than the RNA population average. This revealed that individual isoforms of the COOLAIR transcript adopt multiple structures with different confo

SUBMITTER: Yang M 

PROVIDER: S-EPMC9452300 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature