Spatial profiling of chromatin accessibility in mouse and human tissues.

ABSTRACT: Cellular function in tissue is dependent on the local environment, requiring new methods for spatial mapping of biomolecules and cells in the tissue context¹. The emergence of spatial transcriptomics has enabled genome-scale gene expression mapping^2-5, but the ability to capture spatial epigenetic information of tissue at the cellular level and genome scale is lacking. Here we describe a method for spatially resolved chromatin accessibility profiling of tissue sections using next-generation sequencing (spatial-ATAC-seq) by combining in situ Tn5 transposition chemistry⁶ and microfluidic deterministic barcoding⁵. Profiling mouse embryos using spatial-ATAC-seq delineated tissue-region-specific epigenetic landscapes and identified gene regulators inv

SUBMITTER: Deng Y 

PROVIDER: S-EPMC9452302 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature