T cell receptor fingerprinting enables in-depth characterization of the interactions governing recognition of peptide-MHC complexes.

ABSTRACT: The promiscuous nature of T-cell receptors (TCRs) allows T cells to recognize a large variety of pathogens, but makes it challenging to understand and control T-cell recognition. Existing technologies provide limited information about the key requirements for T-cell recognition and the ability of TCRs to cross-recognize structurally related elements. Here we present a 'one-pot' strategy for determining the interactions that govern TCR recognition of peptide-major histocompatibility complex (pMHC). We measured the relative affinities of TCRs to libraries of barcoded peptide-MHC variants and applied this knowledge to understand the recognition motif, here termed the TCR fingerprint. The TCR fingerprints of 16 different TCRs were identified and used to predict and validate cross-recognized pe

SUBMITTER: Bentzen AK 

PROVIDER: S-EPMC9452375 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature