Liver sinusoidal endothelial cell expressed vascular cell adhesion molecule 1 promotes liver fibrosis.

ABSTRACT:

Background

During liver injury, liver sinusoidal endothelial cells (LSECs) dysfunction and capillarization promote liver fibrosis. We have previously reported that the LSEC vascular cell adhesion molecule 1 (VCAM1) plays a key role in liver inflammation in nonalcoholic steatohepatitis (NASH) and we now aim to uncover its role in LSEC capillarization and liver fibrosis.

Methods

Wild-type C57BL/6J mice were fed either chow or high fat, fructose and cholesterol diet to induce NASH and treated with either anti-VCAM1 neutralizing antibody or control isotype antibody. Inducible endothelial cell-specific Vcam1 deleted mice (Vcam1^Δend ) and control mice (Vcam1^fl/fl ) were fed choline-deficient high-fat diet (CD-HFD) to induce NASH or injected with

SUBMITTER: Guo Q 

PROVIDER: S-EPMC9453231 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature