Extensive nrDNA ITS polymorphism in Lycium: Non-concerted evolution and the identification of pseudogenes.

ABSTRACT: The internal transcribed spacer (ITS) is one of the most extensively sequenced molecular markers in plant systematics due to its generally concerted evolution. While non-concerted evolution has been found in some plant taxa, such information is missing in Lycium. Molecular studies of six species and two variants of the genus Lycium revealed high levels of intra- and inter-individual polymorphism in the ITS, indicating non-concerted evolution. All genomic DNA ITS paralogues were identified as putative pseudogenes or functional paralogues through a series of comparisons of sequence features, including length and substitution variation, GC content, secondary structure stability, and the presence of conserved motifs in the 5.8S gene, and the rate of evolution. Approximately, 60%

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9453804 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature