Metabolic engineering of Halomonas elongata: Ectoine secretion is increased by demand and supply driven approaches.

ABSTRACT: The application of naturally-derived biomolecules in everyday products, replacing conventional synthetic manufacturing, is an ever-increasing market. An example of this is the compatible solute ectoine, which is contained in a plethora of treatment formulations for medicinal products and cosmetics. As of today, ectoine is produced in a scale of tons each year by the natural producer Halomonas elongata. In this work, we explore two complementary approaches to obtain genetically improved producer strains for ectoine production. We explore the effect of increased precursor supply (oxaloacetate) on ectoine production, as well as an implementation of increased ectoine demand through the overexpression of a transporter. Both approaches were implemented on an already genetically modified e

SUBMITTER: Hobmeier K 

PROVIDER: S-EPMC9453808 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature