Estrogen differentially regulates transcriptional landscapes of preoptic and arcuate kisspeptin neuron populations.

ABSTRACT: Kisspeptin neurons residing in the rostral periventricular area of the third ventricle (KP^RP3V) and the arcuate nucleus (KP^ARC) mediate positive and negative estrogen feedback, respectively. Here, we aim to compare transcriptional responses of KP^RP3V and KP^ARC neurons to estrogen. Transgenic mice were ovariectomized and supplemented with either 17β-estradiol (E2) or vehicle. Fluorescently tagged KP^RP3V neurons collected by laser-capture microdissection were subjected to RNA-seq. Bioinformatics identified 222 E2-dependent genes. Four genes encoding neuropeptide precursors (Nmb, Kiss1, Nts, Penk) were robustly, and Cartpt was subsignificantly upregulated, suggesting putative contribution of multiple neuropeptides to estroge

SUBMITTER: Gocz B 

PROVIDER: S-EPMC9454256 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature