Structure of an Intranucleosomal DNA Loop That Senses DNA Damage during Transcription.

ABSTRACT: Transcription through chromatin by RNA polymerase II (Pol II) is accompanied by the formation of small intranucleosomal DNA loops containing the enzyme (i-loops) that are involved in survival of core histones on the DNA and arrest of Pol II during the transcription of damaged DNA. However, the structures of i-loops have not been determined. Here, the structures of the intermediates formed during transcription through a nucleosome containing intact or damaged DNA were studied using biochemical approaches and electron microscopy. After RNA polymerase reaches position +24 from the nucleosomal boundary, the enzyme can backtrack to position +20, where DNA behind the enzyme recoils on the surface of the histone octamer, forming an i-loop that locks Pol II in the arrested state. Since the i-loop

SUBMITTER: Gerasimova NS 

PROVIDER: S-EPMC9454427 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature