Role of Transmembrane Water Exchange in Glioma Invasion/Migration: In Vivo Preclinical Study by Relaxometry at Very Low Magnetic Field.

ABSTRACT: This work shows that the longitudinal relaxation differences observed at very low magnetic fields between invasion/migration and proliferation processes on glioma mouse models in vivo are related to differences in the transmembrane water exchange basically linked to the aquaporin expression changes. Three glioma mouse models were used: Glio6 and Glio96 as invasion/migration models and U87 as cell proliferation model. In vivo proton longitudinal relaxation-rate constants (R₁) at very low fields were measured by fast field cycling NMR (FFC-NMR). The tumor contribution to the observed proton relaxation rate, R₁^tum (U87: 12.26 ± 0.64 s^-1; Glio6: 3.76 ± 0.88 s^-1; Glio96: 6.90 ± 0.64 s^-1 at 0.01 MHz), and the intracellular water l

SUBMITTER: Ruggiero MR 

PROVIDER: S-EPMC9454706 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature