CD34⁺CD10⁺CD19^- Cells in Patients with Unhealthy Alcohol Use Stimulate the M2b Monocyte Polarization.

ABSTRACT: M2b monocytes commonly isolated from patients with unhealthy alcohol use (Alc) have been described as cells that make the host susceptible to opportunistic infections. CD34⁺CD10⁺CD19^- cells are multilineage progenitors of CD19⁺ cells, and we show that the effect of these cells from the peripheral blood on M2b monocyte polarization differed between healthy donors and Alc in this study. In healthy donors, these cells consistently differentiated into high-mobility group box-1 (HMGB1)-nonproducing cells (CD19⁺ cells) in response to retinoic acid (RA). However, owing to the lack of expression of RA receptor (RAR), these cells from Alc failed to differentiate into CD19⁺ cells under the same RA stimulation. Conditioned medium (CM)

SUBMITTER: Asai A 

PROVIDER: S-EPMC9454773 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature