Pulsed Electrical Stimulation Affects Osteoblast Adhesion and Calcium Ion Signaling.

ABSTRACT: An extensive research field in regenerative medicine is electrical stimulation (ES) and its impact on tissue and cells. The mechanism of action of ES, particularly the role of electrical parameters like intensity, frequency, and duration of the electric field, is not yet fully understood. Human MG-63 osteoblasts were electrically stimulated for 10 min with a commercially available multi-channel system (IonOptix). We generated alternating current (AC) electrical fields with a voltage of 1 or 5 V and frequencies of 7.9 or 20 Hz, respectively. To exclude liquid-mediated effects, we characterized the AC-stimulated culture medium. AC stimulation did not change the medium's pH, temperature, and oxygen content. The H₂O₂ level was comparable with the unstimulated samples exce

SUBMITTER: Staehlke S 

PROVIDER: S-EPMC9454840 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature