Dataset Information

Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry-A Perspective Study.

ABSTRACT: Tumor dissemination is one of the most-investigated steps of tumor progression, which in recent decades led to the rapid development of liquid biopsy aiming to analyze circulating tumor cells (CTCs), extracellular vesicles (EVs), and circulating nucleic acids in order to precisely diagnose and monitor cancer patients. Flow cytometry was considered as a method to detect CTCs; however, due to the lack of verification of the investigated cells' identity, this method failed to reach clinical utility. Meanwhile, imaging flow cytometry combining the sensitivity and high throughput of flow cytometry and image-based detailed analysis through a high-resolution microscope might open a new avenue in CTC technologies and provide an open-platform system alternative to CellSearch^®, which is still the only gold standard in this field. Hereby, we shortly review the studies on the usage of flow cytometry in CTC identification and present our own representative images of CTCs envisioned by imaging flow cytometry providing rationale that this novel technology might be a good tool for studying tumor dissemination, and, if combined with a high CTC yield enrichment method, could upgrade CTC-based diagnostics.

SUBMITTER: Muchlinska A

PROVIDER: S-EPMC9454939 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry-A Perspective Study.

Muchlińska Anna A Smentoch Julia J Żaczek Anna J AJ Bednarz-Knoll Natalia N

Cancers 20220829 17

Tumor dissemination is one of the most-investigated steps of tumor progression, which in recent decades led to the rapid development of liquid biopsy aiming to analyze circulating tumor cells (CTCs), extracellular vesicles (EVs), and circulating nucleic acids in order to precisely diagnose and monitor cancer patients. Flow cytometry was considered as a method to detect CTCs; however, due to the lack of verification of the investigated cells' identity, this method failed to reach clinical utility ...[more]

PMID: 36077716

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Project description:Simple Summary Liquid biopsy is a promising but challenging tool for potentially upgrading cancer patient diagnostics and providing new insights into tumor biology. Here, we applied a unique approach to detect CTCs and cCAFs in a one-tube assay using imaging flow cytometry, enabling improved enumeration, multimarker-based phenotyping, and detailed morphological characterization of those rare cells. We identified new, putatively EMT-related phenotypes of negative CTCs for both epithelial and mesenchymal markers and showed that EMT-related CTCs might contribute to breast cancer progression, whereas a coincidence of CTCs and cCAFs might be a signature of metastasis. Abstract Circulating tumor cells (CTCs) and circulating cancer-associated fibroblasts (cCAFs) have been individually considered strong indicators of cancer progression. However, technical limitations have prevented their simultaneous analysis in the context of CTC phenotypes different from epithelial. This study aimed to analyze CTCs and cCAFs simultaneously in the peripheral blood of 210 breast cancer patients using DAPI/pan-keratin (K)/vimentin (V)/alpha-SMA/CD29/CD45/CD31 immunofluorescent staining and novel technology—imaging flow cytometry (imFC). Single and clustered CTCs of different sizes and phenotypes (i.e., epithelial phenotype K+/V− and epithelial–mesenchymal transition (EMT)-related CTCs, such as K+/V+, K−/V+, and K−/V−) were detected in 27.6% of the samples and correlated with metastases. EMT-related CTCs interacted more frequently with normal cells and tended to occur in patients with tumors progressing during therapy, while cCAFs coincided with CTCs (mainly K+/V− and K−/V−) in seven (3.3%) patients and seemed to correlate with the presence of metastases, particularly visceral ones. This study emphasizes the advantages of imFC in the field of liquid biopsy and highlights the importance of multimarker-based analysis of different subpopulations and phenotypes of cancer progression-related cells, i.e., CTCs and cCAFs. The co-detection of CTCs and cCAFs might improve the identification of patients at higher risk of progression and their monitoring during therapy.

Dataset Information

Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry-A Perspective Study.

Publications

Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry-A Perspective Study.

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