Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry-A Perspective Study.

ABSTRACT: Tumor dissemination is one of the most-investigated steps of tumor progression, which in recent decades led to the rapid development of liquid biopsy aiming to analyze circulating tumor cells (CTCs), extracellular vesicles (EVs), and circulating nucleic acids in order to precisely diagnose and monitor cancer patients. Flow cytometry was considered as a method to detect CTCs; however, due to the lack of verification of the investigated cells' identity, this method failed to reach clinical utility. Meanwhile, imaging flow cytometry combining the sensitivity and high throughput of flow cytometry and image-based detailed analysis through a high-resolution microscope might open a new avenue in CTC technologies and provide an open-platform system alternative to CellSearch^®, which is s

SUBMITTER: Muchlinska A 

PROVIDER: S-EPMC9454939 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature