Impact of Growth Conditions on Pseudomonas fluorescens Morphology Characterized by Atomic Force Microscopy.

ABSTRACT: This work is dedicated to the characterization by Atomic Force Microscopy (AFM) of Pseudomonas fluorescens, bacteria having high potential in biotechnology. They were first studied first in optimal conditions in terms of culture medium and temperature. AFM revealed a more-or-less elongated morphology with typical dimensions in the micrometer range, and an organization of the outer membrane characterized by the presence of long and randomly distributed ripples, which are likely related to the organization of lipopolysaccharides (LPS). The outer membrane also presents invaginations, some of them showing a reorganization of ripples, which could be the first sign of a bacterial stress response. In a second step, bacteria grown under unfavorable conditions were characterized. The choice

SUBMITTER: Kahli H 

PROVIDER: S-EPMC9455637 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature