The niche-specialist and age-related oral microbial ecosystem: crosstalk with host immune cells in homeostasis.

ABSTRACT: Although characterization of the baseline oral microbiota has been discussed, the current literature seems insufficient to draw a definitive conclusion on the interactions between the microbes themselves or with the host. This study focuses on the spatial and temporal characteristics of the oral microbial ecosystem in a mouse model and its crosstalk with host immune cells in homeostasis. The V3V4 regions of the 16S rRNA gene of 20 samples from four niches (tongue, buccal mucosa, keratinized gingiva and hard palate) and 10 samples from two life stages (adult and old) were analysed. Flow cytometry (FCM) was used to investigate the resident immune cells. The niche-specialist and age-related communities, characterized based on the microbiota structure, interspecies communications, microbial fu

SUBMITTER: Lin D 

PROVIDER: S-EPMC9455711 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature