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Systemic and Renal Dynamics of Free Sulfhydryl Groups during Living Donor Kidney Transplantation.


ABSTRACT: During ischemia−reperfusion injury (IRI), reactive oxygen species are produced that can be scavenged by free sulfhydryl groups (R-SH, free thiols). In this study, we hypothesized that R-SH levels decrease as a consequence of renal IRI and that R-SH levels reflect post-transplant graft function. Systemic venous, arterial, renal venous, and urinary samples were collected in donors and recipients before, during, and after transplantation. R-SH was measured colorimetrically. Systemic arterial R-SH levels in recipients increased significantly up to 30 sec after reperfusion (p < 0.001). In contrast, renal venous R-SH levels significantly decreased at 5 and 10 min compared to 30 sec after reperfusion (both p < 0.001). This resulted in a significant decrease in delta R-SH (defined as the difference between renal venous and systemic arterial R-SH levels) till 30 sec after reperfusion (p < 0.001), indicating a net decrease in R-SH levels across the transplanted kidney. Overall, these results suggest trans-renal oxidative stress as a consequence of IRI during kidney transplantation, reflected by systemic and renal changes in R-SH levels in transplant recipients.

SUBMITTER: Spraakman NA 

PROVIDER: S-EPMC9455962 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Systemic and Renal Dynamics of Free Sulfhydryl Groups during Living Donor Kidney Transplantation.

Spraakman Nora A NA   Coester Annemieke M AM   Bourgonje Arno R AR   Nieuwenhuijs Vincent B VB   Sanders Jan-Stephan F JF   Leuvenink Henri G D HGD   van Goor Harry H   Nieuwenhuijs-Moeke Gertrude J GJ  

International journal of molecular sciences 20220829 17


During ischemia−reperfusion injury (IRI), reactive oxygen species are produced that can be scavenged by free sulfhydryl groups (R-SH, free thiols). In this study, we hypothesized that R-SH levels decrease as a consequence of renal IRI and that R-SH levels reflect post-transplant graft function. Systemic venous, arterial, renal venous, and urinary samples were collected in donors and recipients before, during, and after transplantation. R-SH was measured colorimetrically. Systemic arterial R-SH l  ...[more]

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