Evaluation of a Serum-Free Medium for Human Epithelial and Stromal Cell Culture

ABSTRACT: Over the past decade, growing demand from many domains (research, cosmetics, pharmaceutical industries, etc.) has given rise to significant expansion of the number of in vitro cell cultures. Despite the widespread use of fetal bovine serum, many issues remain. Among them, the whole constitution of most serums remains unknown and is subject to significant variations. Furthermore, the presence of potential contamination and xenogeny elements is challenging for clinical applications, while limited production is an obstacle to the growing demand. To circumvent these issues, a Serum-Free Medium (SFM) has been developed to culture dermal and vesical fibroblasts and their corresponding epithelial cells, namely, keratinocytes and urothelial cells. To assess the impact of SFM on these cells, prolif

SUBMITTER: Caneparo C 

PROVIDER: S-EPMC9455993 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature