Selective Loading and Variations in the miRNA Profile of Extracellular Vesicles from Endothelial-like Cells Cultivated under Normoxia and Hypoxia.

ABSTRACT: Endothelial-like cells may be obtained from CD133⁺ mononuclear cells isolated from human umbilical cord blood (hUCB) and expanded using endothelial-inducing medium (E-CD133 cells). Their use in regenerative medicine has been explored by the potential not only to form vessels but also by the secretion of bioactive elements. Extracellular vesicles (EVs) are prominent messengers of this paracrine activity, transporting bioactive molecules that may guide cellular response under different conditions. Using RNA-Seq, we characterized the miRNA content of EVs derived from E-CD133 cells cultivated under normoxia (N-EVs) and hypoxia (H-EVs) and observed that changing the O₂ status led to variations in the selective loading of miRNAs in the EVs. In silico analysis showed that am

SUBMITTER: Robert AW 

PROVIDER: S-EPMC9456085 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature