Enhancing the Catalytic Activity of Type II L-Asparaginase from Bacillus licheniformis through Semi-Rational Design.

ABSTRACT: Low catalytic activity is a key factor limiting the widespread application of type II L-asparaginase (ASNase) in the food and pharmaceutical industries. In this study, smart libraries were constructed by semi-rational design to improve the catalytic activity of type II ASNase from Bacillus licheniformis. Mutants with greatly enhanced catalytic efficiency were screened by saturation mutations and combinatorial mutations. A quintuple mutant ILRAC was ultimately obtained with specific activity of 841.62 IU/mg and k_cat/K_m of 537.15 min^-1·mM^-1, which were 4.24-fold and 6.32-fold more than those of wild-type ASNase. The highest specific activity and k_cat/K_m were firstly reported in type II ASNase

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC9456134 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature