The Effect of Trehalose Coating for Magnetite Nanoparticles on Stability of Egg White Lysozyme.

ABSTRACT: In this study, the protein stability of hen egg-white lysozymes (HEWL) by Fe₃O₄ and Fe₃O₄-coated trehalose (Fe₃O₄@Tre) magnetic nanoparticles (NPs) is investigated. For this purpose, the co-precipitation method was used to synthesize magnetic NPs. The synthesized NPs were characterized by XRD, FT-IR spectroscopy, FE-SEM, and VSM analysis. In addition, the stability of HEWLs exposed to different NP concentrations in the range of 0.001-0.1 mg mL^-1 was investigated by circular dichroism (CD) spectroscopy, fluorescence, and UV-Vis analysis. Based on the results, in the NP concentration range of 0.001-0.04 mg mL^-1 the protein structure is more stable, and this range was identified as the range of kosmotropic

SUBMITTER: Lajmorak A 

PROVIDER: S-EPMC9456156 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature