Theoretical and Experimental Assay of Shock Experienced by Yeast Cells during Laser Bioprinting.

ABSTRACT: Laser-induced forward transfer (LIFT) is a useful technique for bioprinting using gel-embedded cells. However, little is known about the stresses experienced by cells during LIFT. This paper theoretically and experimentally explores the levels of laser pulse irradiation and pulsed heating experienced by yeast cells during LIFT. It has been found that only 5% of the cells in the gel layer adjacent to the absorbing Ti film should be significantly heated for fractions of microseconds, which was confirmed by the fact that a corresponding population of cells died during LIFT. This was accompanied by the near-complete dimming of intracellular green fluorescent protein, also observed in response to heat shock. It is shown that microorganisms in the gel layer experience laser irradiation with an e

SUBMITTER: Grosfeld EV 

PROVIDER: S-EPMC9456252 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature