IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation.

ABSTRACT: IL-17A is considered to guide liver inflammation and fibrosis. From twenty-two human liver samples of different fibrosis stages (F0 to F4), IL-17A, IL-22, and TGFβ1 protein expression in liver tissue lysates were analyzed. Ten paired samples of liver tissue (F0-F1 stage) and blood from the same patient were used to analyze intrahepatic and blood T-lymphoid IL-17A⁺ cells by flow cytometry. The analyses have been performed regardless of pathology, considering the stage of fibrosis. Human liver tissue was used for the primary human liver slice cultures, followed by subsequent cytokine stimulation and fibrotic markers' analysis by ELISA. IL-17A production in human liver tissue was significantly higher in the early fibrotic stage compared with the advanced stage. Th17 T cells and, to

SUBMITTER: Kartasheva-Ebertz D 

PROVIDER: S-EPMC9456490 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature