Development and Validation of a Highly Sensitive and Rapid LC-MS³ Strategy to Determine Oxcarbazepine and Its Active Metabolite in the Serum of Patients with Epilepsy and Its Application in Therapeutic Drug Monitoring.

ABSTRACT: A sensitive and rapid bioanalytical method based on the LC-triple-stage fragmentation (LC-MS³) strategy on a hybrid triple quadrupole-linear ion trap mass spectrometer in combination with protein precipitation extraction for sample pretreatment has been developed and validated for the simultaneous determination of the antiepileptic drug oxcarbazepine (OXC) and its main active metabolite (MHD) in human serum. The separation was performed on a Waters XBridge BEH C18 column (2.5 µm, 2.1 × 50 mm) in isocratic elution with 0.1% formic acid in water and methanol (50:50, v:v) as the mobile phase. The run time for each sample was 2.0 min. The calibration curves ranging from 25 to 1600 ng/mL for OXC and from 0.5 to 32 μg/mL for MHD showed correlation coefficients (r) better than 0

SUBMITTER: Ji Z 

PROVIDER: S-EPMC9457704 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature