Dual Inhibition of HIV-1 and Cathepsin L Proteases by Sarcandra glabra.

ABSTRACT: The COVID-19 pandemic continues to impose a huge threat on human health due to rapid viral mutations. Thus, it is imperative to develop more potent antivirals with both prophylactic and treatment functions. In this study, we screened for potential antiviral compounds from Sarcandra glabra (SG) against Cathepsin L and HIV-1 proteases. A FRET assay was applied to investigate the inhibitory effects and UPLC-HRMS was employed to identify and quantify the bioactive components. Furthermore, molecular docking was carried out to get a glimpse of the binding of active compounds to the proteases. Our results showed that the SG extracts (SGW, SG30, SG60, and SG85) inhibited HIV-1 protease with an IC₅₀ of 0.003~0.07 mg/mL and Cathepsin L protease with an IC₅₀

SUBMITTER: Pan B 

PROVIDER: S-EPMC9457736 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature