Quantification of Gut Microbiota Dysbiosis-Related Organic Acids in Human Urine Using LC-MS/MS.

ABSTRACT: Urine organic acid contains water-soluble metabolites and/or metabolites-derived from sugars, amino acids, lipids, vitamins, and drugs-which can reveal a human's physiological condition. These urine organic acids-hippuric acid, benzoic acid, phenylacetic acid, phenylpropionic acid, 4-hydroxybenzoic acid, 4-hydroxyphenyl acetic acid, 3-hydroxyphenylpropionic acid, 3,4-dihydroxyphenyl propionic acid, and 3-indoleacetic acid-were the eligible candidates for the dysbiosis of gut microbiota. The aim of this proposal was to develop and to validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioanalysis method for the nine organic acids in human urine. Stable-labeled isotope standard (creatinine-d₃) and acetonitrile were added to the urine sample. The supernatant was di

SUBMITTER: Lee YT 

PROVIDER: S-EPMC9457824 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature