Differential dependencies of human RNA polymerase II promoters on TBP, TAF1, TFIIB and XPB.

ABSTRACT: The effects of rapid acute depletion of components of RNA polymerase II (Pol II) general transcription factors (GTFs) that are thought to be critical for formation of preinitiation complexes (PICs) and initiation in vitro were quantified in HAP1 cells using precision nuclear run-on sequencing (PRO-Seq). The average dependencies for each factor across >70 000 promoters varied widely even though levels of depletions were similar. Some of the effects could be attributed to the presence or absence of core promoter elements such as the upstream TBP-specificity motif or downstream G-rich sequences, but some dependencies anti-correlated with such sequences. While depletion of TBP had a large effect on most Pol III promoters only a small fraction of Pol II promoters were similarly affected. TFIIB

SUBMITTER: Santana JF 

PROVIDER: S-EPMC9458433 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature