CircMMP9 accelerates the progression of hepatocellular carcinoma through the miR-149/CCND2 axis.

ABSTRACT:

Background

This study aimed to verify the hypothesis that circular RNA MMP9 (circMMP9) promotes hepatocellular carcinoma (HCC) progression through targeting miR-149 and regulating cyclin D2 (CCND2) expression.

Methods

Expression of circMMP9, miR-149 and CCND2 was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) or protein blotting. Cell Counting Kit-8 (CCK-8) was used to evaluate cell proliferation. The HCC cell migration and invasion were evaluated using wound healing and transwell assays. The interaction among circMMP9, miR-149, and CCND2 was evaluated using luciferase, RNA-pull down, and RNA immunoprecipitation (RIP) assays, respectively. Cell apoptosis and cycle were e

SUBMITTER: Li X 

PROVIDER: S-EPMC9459201 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature