METTL3-Mediated m⁶A Modification of lncRNA MALAT1 Facilitates Prostate Cancer Growth by Activation of PI3K/AKT Signaling.

ABSTRACT: Accumulating data show that N6-methyladenosine (m⁶A) methyltransferase METTL3 and long noncoding RNA MALAT1 act pivotal roles in multiple malignancies including prostate cancer (PCa). However, the role and molecular mechanism underlying METTL3-mediated m⁶A modification of MALAT1 in PCa remain undocumented. The association of METTL3 and MALAT1 expression with clinicopathological characteristics and prognosis in patients with PCa was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, and public The Cancer Genome Atlas (TCGA) dataset. The in vitro and in vivo experiments were executed to investigate the role of METTL3 in PCa. m⁶A dot blot, methylated RNA immunoprecipitation (MeRIP), RIP, and qRT-PCR assays were

SUBMITTER: Mao Y 

PROVIDER: S-EPMC9459491 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature