Propranolol suppresses bladder cancer by manipulating intracellular pH via NHE1.

ABSTRACT:

Background

In recent years, a large number of clinical and epidemiological studies have revealed the anti-cancer activity of propranolol in solid tumors, though the underline mechanism is yet to be clarified.

Methods

The proliferation of bladder cancer cells treated with propranolol was detected by MTS assays. In vivo tumor xenograft experiments were used to observe the effect of propranolol on bladder cancer growth in mice. The expression levels of Na⁺/H⁺ exchanger (NHE1) was measured by western blot. The frequency of CD8⁺ T cells and CD4⁺ T cells were detected via flow cytometry.

Results

In this study, propranolol inhibited the expression of NHE1 and sequentially led to a decrease of intracellular pH to 5.88 in MB49

SUBMITTER: Hu Q 

PROVIDER: S-EPMC9459546 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature