Global chromatin accessibility profiling analysis reveals a chronic activation state in aged muscle stem cells.

ABSTRACT: Regulation of chromatin accessibility is critical for cell fate decisions. Chromatin structure responds to extrinsic environments rapidly. The traditional adult stem cell isolation approach requires tissue dissociation, which triggers stem cell activation and leads to alterations in chromatin structure. To preserve the in vivo chromatin states, we utilized the PFA-perfusion-based isolation approach and characterized the DNA regulatory landscapes during muscle stem cell quiescence exit and aging. We showed that aged SCs display a chronically activated chromatin signature. Detailed analysis of the chromatin accessibility profiles identified key enhancer elements for SC quiescence. Constant activation of the enhancer elements promotes stemness and prevents SCs from differentiation, whe

SUBMITTER: Dong A 

PROVIDER: S-EPMC9459695 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature