Establishment of an Improved ELONA Method for Detecting Fumonisin B₁ Based on Aptamers and Hemin-CDs Conjugates.

ABSTRACT: Fumonisin B₁ (FB₁) is a strong mycotoxin that is ubiquitous in agricultural products. The establishment of rapid detection methods is an important means to prevent and control FB₁ contamination. In this study, an improved enzyme-linked oligonucleotide assay (ELONA) method was designed and tested to detect the contents of FB₁ in maize (corn) samples. F10 modified with biotin was bound to an enzyme label plate that was coated with streptavidin (SA) in advance, and carbon dots (CDs) were used to catalyze the color of tetramethylbenzidine (TMB). The complementary chain of F10 was modified with an amino group and coupled with CDs to obtain conjugates. The sample and conjugates were then added to the enzyme plate coated with F10 (an FB₁ apta

SUBMITTER: Zhao X 

PROVIDER: S-EPMC9460299 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature