Quantification of Serum Exosome Biomarkers Using 3D Nanoporous Gold and Spectrophotometry.

ABSTRACT: Tumor-derived exosomes may provide biomarkers for cancer treatment. Using sputtering technology, an affinity-based device to capture exosomes was developed using nanoporous substrate (NPG)-coated silicon microscopy. Immunology-based techniques detect and purify exosomes using gold coating with a specific antigen. Inverted fluorescent microscopy was used to detect target exosomes quantitatively utilizing fluorescent nanospheres as the label. We quantified the expression of CD63 surface protein markers on exosomes from conditioned culture media of breast cancer cells. The exosomes that targeted specific proteins with controls were statistically analyzed and compared to those that targeted non-specific proteins. Results from SEM showed that the exosomes were circular, between 30 and 150 nanom

SUBMITTER: Al Mannai A 

PROVIDER: S-EPMC9460504 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature