Studies on the Reaction of Dietary Methylglyoxal and Creatine during Simulated Gastrointestinal Digestion and in Human Volunteers.

ABSTRACT: The reactive 1,2-dicarbonyl compound methylglyoxal (MGO) is consumed with food and its concentrations decrease during digestion. In the present paper, the reaction of MGO with creatine, arginine, and lysine during simulated digestion, and its reaction with creatine during the digestion in human volunteers, was studied. Therefore, simulated digestion experiments with a gastric and an intestinal phase were performed. Additionally, an intervention study with 12 subjects consuming MGO-containing Manuka honey and creatine simultaneously or separately was conducted. Derivatization with o-phenylenediamine and HPLC-UV was used to measure MGO, while creatine and glycated amino compounds were analyzed via HPLC-MS/MS. We show that MGO quickly reacts with creatine and arginine, but not lysine, during

SUBMITTER: Treibmann S 

PROVIDER: S-EPMC9460645 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature