Customizable Collagen Vitrigel Membranes and Preliminary Results in Corneal Engineering.

ABSTRACT: Corneal opacities are a leading cause of visual impairment that affect 4.2 million people annually. The current treatment is corneal transplantation, which is limited by tissue donor shortages. Corneal engineering aims to develop membranes that function as scaffolds in corneal cell transplantation. Here, we describe a method for producing transplantable corneal constructs based on a collagen vitrigel (CVM) membrane and corneal endothelial cells (CECs). The CVMs were produced using increasing volumes of collagen type I: 1X (2.8 μL/mm²), 2X, and 3X. The vitrification process was performed at 40% relative humidity (RH) and 40 °C using a matryoshka-like system consisting of a shaking-oven harboring a desiccator with a saturated K₂CO₃ solution. The CVMs were cha

SUBMITTER: Montalvo-Parra MD 

PROVIDER: S-EPMC9460691 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature