ROS attenuates TET2-dependent ZO-1 epigenetic expression in cerebral vascular endothelial cells

ABSTRACT:

Aims

To investigate whether DNA active demethylase TET regulates the expression of tight junction proteins in endothelial cells of the blood–brain barrier (BBB).

Methods

Correlations between TET2 activity (indicated by its catalytic product 5hmC) and the expression of BBB tight junction proteins were examined in Tet2 knockout mice and post-mortem human brain tissues. In cultured endothelial cells, the impact of changes of TET activity on the expression of tight junction protein, ZO-1, was studied. BBB permeability assays were performed in Tet2 knockout mice.

Results

It was found that the level of 5hmC decreased in brain microvascular endothelial cells of aging mice. In Tet2 knockout mice, the level of 5hmC in endothelial cells was weaker and significantly correla

SUBMITTER: Wang L 

PROVIDER: S-EPMC9461112 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature