Discovery and characterization of heterogeneous and multipotent fibroblast populations isolated from excised cleft lip tissue.

ABSTRACT:

Background

Regularly discarded lip tissue obtained from corrective surgeries to close the cleft lip represents an easily accessible and rich source for the isolation of primary fibroblasts. Primary fibroblasts have been described to show compelling similarities to mesenchymal stem cells (MSCs). Hence, cleft lip and palate (CLP) lip-derived fibroblasts could be thought as an intriguing cell source for personalized regenerative therapies in CLP-affected patients.

Methods

Initially, we thoroughly characterized the fibroblastic nature of the lip-derived mesenchymal outgrowths by molecular and functional assays. Next, we compared their phenotype and genotype to that of bone marrow-mesenchymal stem cells (BM-MSCs) and of human lung-derived fibroblasts WI38, by assessing their morp

SUBMITTER: Parisi L 

PROVIDER: S-EPMC9461253 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature