TSLP promoting B cell proliferation and polarizing follicular helper T cell as a therapeutic target in IgG4-related disease.

ABSTRACT:

Objective

To figure out the functions of thymic stromal lymphopoietin (TSLP) in IgG4-related disease (IgG4-RD).

Methods

Plasma TSLP levels were tested by Elisa, and its receptors were detected by flow cytometry. Expressions of TSLP and TSLPR in involved tissues were stained by immunohistochemistry and immunofluorescence. Proliferation, apoptosis, and B subsets of TSLP stimulated-B cells were analyzed by flow cytometry. TSLP-stimulated B cells were co-cultured with CD4+ Naïve T cells. Signaling pathway was identified by RNA-sequencing and western blot. Anti-TSLP therapy was adapted in Lat^Y136F knock-in mice (Lat, IgG4-RD mouse model).

Results

Plasma TSLP level was increased in IgG4-RD patients and was positively correlated with serum IgG4 level and responder

SUBMITTER: Lu H 

PROVIDER: S-EPMC9461269 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature