Method for reproducible automated bacterial cell culture and measurement.
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ABSTRACT:
SUBMITTER: Ross D
PROVIDER: S-EPMC9462466 | biostudies-literature | 2022
REPOSITORIES: biostudies-literature
Publications
Method for reproducible automated bacterial cell culture and measurement.
Ross David D Tonner Peter D PD Vasilyeva Olga B OB
Synthetic biology (Oxford, England) 20220810 1
Microbial cell culture is one of the most commonly performed protocols for synthetic biology, and laboratories are increasingly using 96-well plates and laboratory automation systems for cell culture. Here, we describe a method for reproducible microbial culture using laboratory automation systems, including automated liquid handling, automated plate sealing and de-sealing, automated incubation and measurement of growing cultures. We discuss the key considerations that, in our experience, are im ...[more]
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