An LED-Based structured illumination microscope using a digital micromirror device and GPU accelerated image reconstruction.

ABSTRACT: When combined with computational approaches, fluorescence imaging becomes one of the most powerful tools in biomedical research. It is possible to achieve resolution figures beyond the diffraction limit, and improve the performance and flexibility of high-resolution imaging systems with techniques such as structured illumination microscopy (SIM) reconstruction. In this study, the hardware and software implementation of an LED-based super-resolution imaging system using SIM employing GPU accelerated parallel image reconstruction is presented. The sample is illuminated with two-dimensional sinusoidal patterns with various orientations and lateral phase shifts generated using a digital micromirror device (DMD). SIM reconstruction is carried out in frequency space using parallel CUDA kernel fu

SUBMITTER: Aydın M 

PROVIDER: S-EPMC9462783 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature