Multiscale study of the oral and gut environments in children with high- and low-threshold peanut allergy.

ABSTRACT:

Background

The oral and gut microbiomes have each been associated with food allergy status. Within food allergy, they may also influence reaction thresholds.

Objective

Our aim was to identify oral and gut microbiota associated with reaction thresholds in peanut allergy.

Methods

A total of 59 children aged 4 to 14 years with suspected peanut allergy underwent double-blind, placebo-controlled food challenge to peanut. Those children who reacted at the 300-mg or higher dose of peanut were classified as high-threshold (HT), those who reacted to lower doses were classified as low-threshold (LT), and those children who did not react were classified as not peanut allergic (NPA). Saliva and stool samples collected before challenge underwent DNA isolation followed by 16S rRNA

SUBMITTER: Zhang L 

PROVIDER: S-EPMC9463091 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature