NMR as a readout to monitor and restore the integrity of complex chemoenzymatic reactions.

ABSTRACT: The non-invasive nature of NMR offers a means to monitor biochemical reactions in situ at the atomic level. We harness this advantage to monitor a complex chemoenzymatic reaction that sequentially modifies reagents and loads the product on a nonribosomal peptide synthetase carrier protein. We present a protocol including a pulse sequence that permits to assess both the integrity of reagents and the completion of each step in the reaction, thus alleviating otherwise time-consuming and costly approaches to debug and repeat inefficient reactions. This study highlights the importance of NMR as a tool to establish reliable and reproducible experimental conditions in biochemical studies.

SUBMITTER: Marincin KA 

PROVIDER: S-EPMC9463103 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature