Role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction.

ABSTRACT: In the current study, we explored the role of Mg²⁺-doped CaSO₄/β-TCP composite biopolymer in regulating macrophage polarization and its relation with enhanced osteogenic differentiation of periodontal ligament stem cells. Furthermore, mechanism underling the regulation of macrophage polarization by CaSO₄/β-TCP was evaluated. Mg²⁺-doped CaSO₄/β-TCP composite was characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). Macrophage polarization was characterized using flow cytometry analysis. Macrophage morphometric analysis was conducted by FITC phalloidin staining. Western blot and qRT-PCR assays were used to assess gene expression levels and miRNAs, respectively. SEM morphology of CaSO₄/β-TCP ceramic r

SUBMITTER: Zhou J 

PROVIDER: S-EPMC9463206 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature