Silencing Itch in human peripheral blood monocytes promotes their differentiation into osteoclasts.

ABSTRACT:

Introduction

Two clinical case reports of humans with mutations in Itch reported distinct morphological defects such as stunted growth, macrocephaly, and dysmorphic features indicating a role for Itch in bone remodelling. Studies in mice have found that the encoded E3 ubiquitin ligase acts as a negative regulator of osteoclastogenesis, however no studies have investigated whether this is translatable to a human model.

Experimental procedures

Human peripheral blood monocytes were separated from whole blood and grown in M-CSF containing media. Media was later supplemented with RANKL to promote osteoclast differentiation. Transient siRNA-mediated Itch knockdown (si-Itch) in monocytes was verified by qPCR and western blot to confirm reduction in both Itch mRNA and protein respec

SUBMITTER: Read OJ 

PROVIDER: S-EPMC9463264 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature