Traject3d allows label-free identification of distinct co-occurring phenotypes within 3D culture by live imaging.

ABSTRACT: Single cell profiling by genetic, proteomic and imaging methods has expanded the ability to identify programmes regulating distinct cell states. The 3-dimensional (3D) culture of cells or tissue fragments provides a system to study how such states contribute to multicellular morphogenesis. Whether cells plated into 3D cultures give rise to a singular phenotype or whether multiple biologically distinct phenotypes arise in parallel is largely unknown due to a lack of tools to detect such heterogeneity. Here we develop Traject3d (Trajectory identification in 3D), a method for identifying heterogeneous states in 3D culture and how these give rise to distinct phenotypes over time, from label-free multi-day time-lapse imaging. We use this to characterise the temporal landscape of morphological s

SUBMITTER: Freckmann EC 

PROVIDER: S-EPMC9463449 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature