DNA Origami-Platelet Adducts: Nanoconstruct Binding without Platelet Activation.

ABSTRACT: Objective. Platelets are small, mechanosensitive blood cells responsible for maintaining vascular integrity and activatable on demand to limit bleeding and facilitate thrombosis. While circulating in the blood, platelets are exposed to a range of mechanical and chemical stimuli, with the platelet membrane being the primary interface and transducer of outside-in signaling. Sensing and modulating these interface signals would be useful to study mechanochemical interactions; yet, to date, no methods have been defined to attach adducts for sensor fabrication to platelets without triggering platelet activation. We hypothesized that DNA origami, and methods for its attachment, could be optimized to enable nonactivating instrumentation of the platelet membrane. Approach and Results.

SUBMITTER: Roka-Moiia Y 

PROVIDER: S-EPMC9463463 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature