Novel assays for quality evaluation of XueBiJing: Quality variability of a Chinese herbal injection for sepsis management.

ABSTRACT: XueBiJing is an intravenous five-herb injection used to treat sepsis in China. The study aimed to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS)- or liquid chromatography-ultraviolet (LC-UV)-based assay for quality evaluation of XueBiJing. Assay development involved identifying marker constituents to make the assay therapeutically relevant and building a reliable one-point calibrator for monitoring the various analytes in parallel. Nine marker constituents from the five herbs were selected based on XueBiJing's chemical composition, pharmacokinetics, and pharmacodynamics. A selectivity test (for "similarity of response") was developed to identify and minimize interference by non-target constituents. Then, an intercept test was developed to fulfill "linearity through zer

SUBMITTER: Yu X 

PROVIDER: S-EPMC9463487 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature