High hydrostatic pressure induces slow contraction in mouse cardiomyocytes.

ABSTRACT: Cardiomyocytes are contractile cells that regulate heart contraction. Ca²⁺ flux via Ca²⁺ channels activates actomyosin interactions, leading to cardiomyocyte contraction, which is modulated by physical factors (e.g., stretch, shear stress, and hydrostatic pressure). We evaluated the mechanism triggering slow contractions using a high-pressure microscope to characterize changes in cell morphology and intracellular Ca²⁺ concentration ([Ca²⁺]_i) in mouse cardiomyocytes exposed to high hydrostatic pressures. We found that cardiomyocytes contracted slowly without an acute transient increase in [Ca²⁺]_i, while a myosin ATPase inhibitor interrupted pressure-induced slow contractions. Furthermore, transmission electron micr

SUBMITTER: Yamaguchi Y 

PROVIDER: S-EPMC9463647 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature