Heterogeneous nanoscopic lipid diffusion in the live cell membrane and its dependency on cholesterol.

ABSTRACT: Cholesterol plays a unique role in the regulation of membrane organization and dynamics by modulating the membrane phase transition at the nanoscale. Unfortunately, due to their small sizes and dynamic nature, the effects of cholesterol-mediated membrane nanodomains on membrane dynamics remain elusive. Here, using ultrahigh-speed single-molecule tracking with advanced optical microscope techniques, we investigate the diffusive motion of single phospholipids in the live cell plasma membrane at the nanoscale and its dependency on the cholesterol concentration. We find that both saturated and unsaturated phospholipids undergo anomalous subdiffusion on the length scale of 10-100 nm. The diffusion characteristics exhibit considerable variations in space and in time, indicating that the nanoscop

SUBMITTER: Chai YJ 

PROVIDER: S-EPMC9463655 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature