Selection of DNA aptamer and its application as an electrical biosensor for Zika virus detection in human serum.

ABSTRACT: Zika virus is a highly infectious virus that is part of the flavivirus group. Precise diagnosis of the Zika virus is significant issue for controlling a global pandemic after the COVID-19 era. For the first time, we describe a zika virus aptamer-based electrical biosensor for detecting Zika virus in human serum. The electrical biosensor composed of a Zika virus aptamer/MXene nanoparticle heterolayer on Au micro-gap electrode (AuMGE)/print circuit board (PCB) system. The Zika virus aptamer was designed to bind the envelope protein of the Zika virus by systematic evolution of ligands by exponential enrichment (SELEX) technique. The binding affinity of the aptamer was determined by fluorescence. For improving the sensor signal sensitivity, Ti₃C₂T_x MXene was in

SUBMITTER: Park G 

PROVIDER: S-EPMC9463662 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature